WormBase Tree Display for Construct: WBCnstr00011057
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| WBCnstr00011057 | Other_name | Expr2978_Ex | |
|---|---|---|---|
| Summary | [nac-2::gfp] | ||
| Driven_by_gene | WBGene00003518 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Transcriptional_fusion | ||
| Construction_summary | [nac-2::gfp] transcriptional fusion. A DNA fragment (~2.1 kb) containing the C. elegans nac-2 gene promoter region was generated by PCR using this cosmid DNA as the template. The sense primer (co-ordinates in cosmid R107 are 11,660 to 11,630) with a SalI adapter attached to the 5'-end was 5'-GTC GAC GAG GTG TTA AAC TGT ATA GTC GTG GTG-3' and the reverse primer (co-ordinates in cosmid R107 are 9609 to 9637) with a BamHI adapter attached to the 5'-end was 5'-GCC GGA TCC AAG AAG TAC CAG AAG CTT TTT TAT-3'. This promoter element was then inserted into the GFP-expression vector pPD117.01 at a SalI/BamHI site upstream of the GFP coding sequence. --precise ends. | ||
| Clone | R107 | ||
| pPD117.01 | |||
| Used_for | Transgene_construct | WBTransgene00028283 | |
| Reference | WBPaper00006504 |
