WormBase Tree Display for Construct: WBCnstr00011026
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| WBCnstr00011026 | Other_name | Expr2932_Ex | |
|---|---|---|---|
| Summary | [pro-1::gfp] | ||
| Driven_by_gene | WBGene00004185 | ||
| Gene | WBGene00004185 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [pro-1::gfp] translational fusion. pGC29 (pro-1 promoter driving GFP): (1) 3318 bp upstream of pro-1 (including the ATG) was amplified from pGC16 (attB1, attB2 sites in the primers) and recombined into attP1, attP2 sites in pDONR221 (Invitrogen) to make pGC22. pGC22 was recombined with pPD117.01GtwyGFP (gift of B. Grant) that contains a Gateway donor cassette (attR1, attR2 in reading frame B ligated into a blunted Asp718 site) upstream of GFP and the let-858 3UTR. pGC29 and pRF4 were injected at 100 N5g/ml each to generate lines. --precise ends. | ||
| Clone | pRF4 | ||
| pGC22 | |||
| Used_for | Transgene_construct | WBTransgene00028252 | |
| Reference | WBPaper00006484 |
