WormBase Tree Display for Construct: WBCnstr00010988
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| WBCnstr00010988 | Other_name | Expr2854_Ex | |
|---|---|---|---|
| Summary | [sel-7::gfp] | ||
| Driven_by_gene | WBGene00004764 | ||
| Gene | WBGene00004764 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [sel-7::gfp] translational fusion. SEL-7::GFP fusion protein, tagged at the amino-terminus, expressed from sel-7 genomic sequence. sel-7 cDNA was obtained. A SalI site was generated before the stop codon by PCR with a pair of complementary primers (5' -GACCCAAGTCTATTCAAGGTTGTCGACTAAAATCCTCGCCTAGC- 3'). GFP was excised from a KSGFPS65T vector (pPD114.38) with SalI and inserted in frame into the cDNA construct. The 5 untranslated region of sel-12 was excised with SacI and NotI from the vector superPin2 (a modified version of Pin2, which contains the whole genomic region of sel-12, and then inserted before the sel-7 cDNA::gfp construct. --precise ends. | ||
| Clone | pPD114.38 | ||
| Used_for | Transgene_construct | WBTransgene00028214 | |
| Reference | WBPaper00006438 |
