WormBase Tree Display for Construct: WBCnstr00010817
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WBCnstr00010817 | Other_name | Expr2414_Ex | |
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Summary | [crt-1::gfp] | ||
Driven_by_gene | WBGene00000802 | ||
Fusion_reporter | GFP | ||
Construction_summary | [crt-1::gfp]. To construct the pcrt-1GFP reporter gene, a 1479 bp fragment of crt-1 promoter sequences (corresponding to sequences between 45353 and 46814 in the Y38A10A cosmid, accession number AF125963) was amplified from N2 genomic DNA using primers 5 -GCAGCCCGGGTTCATTTCGTTTTTCGATA-3 and 5 -AGATCTGCAGGTGAACCGAGCCCGAGTAG-3 , digested with PstI and SmaI, then ligated into GFP vector pPD95.77. --precise ends. | ||
Clone | Y38A10A | ||
Used_for | Transgene_construct | WBTransgene00028046 | |
Reference | WBPaper00004880 |