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WormBase Tree Display for Construct: WBCnstr00010817

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Name Class

WBCnstr00010817Other_nameExpr2414_Ex
Summary[crt-1::gfp]
Driven_by_geneWBGene00000802
Fusion_reporterGFP
Construction_summary[crt-1::gfp]. To construct the pcrt-1GFP reporter gene, a 1479 bp fragment of crt-1 promoter sequences (corresponding to sequences between 45353 and 46814 in the Y38A10A cosmid, accession number AF125963) was amplified from N2 genomic DNA using primers 5 -GCAGCCCGGGTTCATTTCGTTTTTCGATA-3 and 5 -AGATCTGCAGGTGAACCGAGCCCGAGTAG-3 , digested with PstI and SmaI, then ligated into GFP vector pPD95.77. --precise ends.
CloneY38A10A
Used_forTransgene_constructWBTransgene00028046
ReferenceWBPaper00004880