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WormBase Tree Display for Construct: WBCnstr00010459

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Name Class

WBCnstr00010459Other_nameExpr1801_Ex
Summary[H30A04.1::gfp]
Driven_by_geneWBGene00001148
GeneWBGene00001148
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[H30A04.1::gfp] translational fusions. To construct GFP reporters, promoter fragments were amplified from wildtype (N2) genomic DNA using gene specific oligonucleotides. Each oligonucleotide pair was engineered with unique restriction sites to facilitate cloning of the promoter fragments into the GFP::HIS2B reporter vector pAP.10 (which was built from a GFP::HIS2B reporter kindly provided by G. Seydoux). GFP was fused in-frame to coding sequence in the fourth or fifth exon.
Used_forTransgene_constructWBTransgene00027695
ReferenceWBPaper00005056