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WormBase Tree Display for Construct: WBCnstr00010458

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Name Class

WBCnstr00010458Other_nameExpr1800_Ex
Summary[peb-1::gfp]
Driven_by_geneWBGene00003968
GeneWBGene00003968
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[peb-1::gfp] translational fusion. To construct GFP reporters, promoter fragments were amplified from wildtype (N2) genomic DNA using gene specific oligonucleotides. Each oligonucleotide pair was engineered with unique restriction sites to facilitate cloning of the promoter fragments into the GFP::HIS2B reporter vector pAP.10 (which was built from a GFP::HIS2B reporter kindly provided by G. Seydoux). GFP was fused in-frame to coding sequence in the second exon.
Used_forTransgene_constructWBTransgene00027694
ReferenceWBPaper00005056