WormBase Tree Display for Construct: WBCnstr00010458
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WBCnstr00010458 | Other_name | Expr1800_Ex | |
---|---|---|---|
Summary | [peb-1::gfp] | ||
Driven_by_gene | WBGene00003968 | ||
Gene | WBGene00003968 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [peb-1::gfp] translational fusion. To construct GFP reporters, promoter fragments were amplified from wildtype (N2) genomic DNA using gene specific oligonucleotides. Each oligonucleotide pair was engineered with unique restriction sites to facilitate cloning of the promoter fragments into the GFP::HIS2B reporter vector pAP.10 (which was built from a GFP::HIS2B reporter kindly provided by G. Seydoux). GFP was fused in-frame to coding sequence in the second exon. | ||
Used_for | Transgene_construct | WBTransgene00027694 | |
Reference | WBPaper00005056 |