WormBase Tree Display for Construct: WBCnstr00010351
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| WBCnstr00010351 | Other_name | Expr1614_Ex | |
|---|---|---|---|
| Summary | [cdh-3::lacZ] | ||
| Driven_by_gene | WBGene00000395 | ||
| Fusion_reporter | GFP | ||
| LacZ | |||
| Construction_summary | [cdh-3::lacZ] or [cdh-3::gfp] constructs that placed lacZ and GFP reporter genes under the control of 6.0 kb of cdh-3 upstream sequences. A number of independent transgenic lines were generated for each construct; all gave identical patterns of expression, differing only in their intracellular localization. Sequences placed between the cdh-3-derived part of each construct and the reporter gene determined the intracellular localization of the reporter protein. To determine the expression pattern in embryos pJP#19 was used, which produces a nuclear localized cdh-3-beta-galactosidase fusion protein, since it was easier to identify cells within the restricted space of the eggshell. Throughout the rest of postembryonic development cdh-3::GFP construct pJP#38 was used. | ||
| Used_for | Transgene_construct | WBTransgene00027590 | |
| Reference | WBPaper00002636 |
