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WormBase Tree Display for Construct: WBCnstr00009460

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Name Class

WBCnstr00009460Summary[PQN-47::GFP; pttx-3::dsRed]
Driven_by_geneWBGene00004134
GeneWBGene00004134
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summaryThis construct contains all endogenous genomic features; the full promoter to upstream gene F59B10.2, introns, and full endogenous 3'UTR. The 5' fragment contains the promoter and first two and half exons from PCR of genomic DNA using oligos 1stpartexon3pqn-47low agcagttcccggttggctc and promoterpqn-47-7124low attgctaaagccatcagaggg. The 3' fragment is the C terminal part of pqn-47 (2nd half of exon 3 thru the 3'UTR) amplified from genomic DNA using oligos: 2ndpartexon3pqn-47up gcagtcaatcaacctacaaaca and after3utrpqn 47low aaacatcacaggtacaatcgg. GFP was amplified from pD95_79 with oligos: gfptopqnlow tgtttgtaggttgattgactgctttgtatagttcatccatgcc and gfptopqnup gagccaaccgggaactgctatgagtaaaggagaagaacttttc.
Used_forTransgene_constructWBTransgene00009798
InteractorWBInteraction000505088
WBInteraction000505089
WBInteraction000505090
WBInteraction000505091
WBInteraction000505146
WBInteraction000505147
WBInteraction000505148
WBInteraction000505149
ReferenceWBPaper00040293