WormBase Tree Display for Construct: WBCnstr00008319
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| WBCnstr00008319 | Public_name | pJG01 | |
|---|---|---|---|
| Summary | [nhr-80p::nhr-80::gfp] | ||
| Driven_by_gene | WBGene00003670 | ||
| Gene | WBGene00003670 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | Clone = pJG01. This construct contains nhr-80 tagged with GFP driven by the endogenous nhr-80 promoter and was constructed by amplifying genomic DNA from 1.4 kb upstream from the start codon until the end of the nhr-80 coding sequence without the stop codon. The 4kb XmaI/KpnI nhr-80 fragment generated was inserted upstream and in frame of the GFP sequence in the worm expression vector pPD95.75 (Fire Lab Vector Kit-Addgene). Primer sequences: Nhr80-XmaI-F: 5'-GGGGTGCCCCCGGGGGGATCGAGACACTTTTCTTACTCCTC-3' Nhr80-KpnI-R: 5'-AACGGGGTACCCCGTTTTTCAAGCTTTGCCTGACCCA-3'. | ||
| Used_for | Transgene_construct | WBTransgene00008615 | |
| Interactor | WBInteraction000504781 | ||
| Reference | WBPaper00038251 |
