WormBase Tree Display for Construct: WBCnstr00005429
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| WBCnstr00005429 | Summary | [aex-2::aex-2-mCherry] | |
|---|---|---|---|
| Driven_by_gene | WBGene00000085 | ||
| Gene | WBGene00000085 | ||
| Fusion_reporter | mCherry | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | Fosmid T14B1.2 containing aex-2 sequence was transformed into recombineering strain SW102. A galK gene was inserted into the C -terminus of the aex-2 coding region with following oligonucleotides: forward GACGAGCATCTGAAAGGCCGCCGGAGCACACCCCCTTACGGTGTGATATGCCTGTTGACAATTAATCATCGGCA, reverse CATTTTTTCCACAAGTTTTACTTACATACATTGCGAATTACTACGATCTATCAGCACTGTCCTGCTCCTT. The galK gene was subsequently replaced by the mCherry gene by homologous recombination with the following oligonucleotides: forward GACGAGCATCTGAAAGGCCGCCGGAGCACACCCCCTTACGGTGTGATATGATGGTGAGCAAGGGCGAGGAG, reverse CATTTTTTCCACAAGTTTTACTTACATACATTGCGAATTACTACGATCTACTTGTACAGCTCGTCCATGCC. In the final step, the whole fragment, which contains aex-2 promoter, aex-2 coding sequence, mCherry coding sequence, and 3'-UTR, was gap repaired into an Amp containing vector backbone using oligonucleotides for ward CATTGATCTGCCGCATGATGAAGTACCAAGTCTGAATGATGAAGAATTTCATTCGTTATGCATTATGGGTAC and reverse AATCAAACGACATTAACGATTTCTCAAAAAAAAAAAACTTTAGGAAAACATACCAATCTAAGTCTGTGCTCC and was used to make transgenes jsEx937 and jsEx938. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00005508 | |
| Reference | WBPaper00032261 |
