WormBase Tree Display for Construct: WBCnstr00005027
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| WBCnstr00005027 | Summary | [pie-1p::GFP-csnk-1 unc-119(+)] | |
|---|---|---|---|
| Driven_by_gene | WBGene00004027 | ||
| Gene | WBGene00013709 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | For generation of GFP::CSNK-1 transgenic animals, full-length CSNK-1 was amplified from Y. Kohara cDNA yk610d10 and cloned into pID2.02 (containing unc-119(+)) according to the manufacturer's protocol (Gateway cloning technology, Invitrogen). The primers used were 5'-GGGGACAAGTTTCTACAAAAAAGCAGGCTTGACGAACACACGCGGGA-3' and 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTCCTATTTTTGTGTAGCTGGGGTCGCATT-3'. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00005050 | |
| Reference | WBPaper00032096 |
