WormBase Tree Display for Construct: WBCnstr00004702
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| WBCnstr00004702 | Public_name | pRL475 | |
|---|---|---|---|
| Summary | [oma-1p::oma-1::GFP] | ||
| Driven_by_gene | WBGene00003864 | ||
| Gene | WBGene00003864 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | The 6-kb rescuing genomic sequence containing the entire OMA-1 coding sequence, 2.1 kb upstream, and 2.7 kb downstream coding sequences was cloned into the Bluescript pSK. An SfiI site was engineered by site directed mutagenesis at the last nucleotide of oma-1 coding region (pRL472). The gfp sequence was PCR amplified from pPD104.53 with primers each containing an SfiI site and subcloned into the SfiI site of pRL472 resulting in pRL475. | ||
| Used_for | Transgene_construct | WBTransgene00004720 | |
| Reference | WBPaper00005930 | ||
| WBPaper00006085 | |||
| WBPaper00026970 | |||
| WBPaper00027607 | |||
| WBPaper00032030 | |||
| WBPaper00041239 | |||
| WBPaper00044909 | |||
| WBPaper00044960 |
