WormBase Tree Display for Construct: WBCnstr00001810
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| WBCnstr00001810 | Summary | [pie-1::GFP-cav-1] | |
|---|---|---|---|
| Driven_by_gene | WBGene00004027 | ||
| Gene | WBGene00000301 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | CAV-1 was expressed with an amino- or carboxy-terminal GFP tag in the C. elegans germline using the germ line-specific pie-1 promoter. Identical expression patterns were obtained by the two constructs. To create a carboxy-terminal GFP fusion with CAV-1, a genomic fragment containing the ORF in cav-1 was amplified by PCR with cav-1Gw+ and cav-1Bgl2R [5'-GGGGACAACTTTGTACAAGAAAGTTGTTAagatctGACGCATGGAGCAGTAGTTTC-3'] and cloned into the Entry vector pDONR221, resulting pDONR221cav-1BglII. A DNA fragment encoding GFP was amplified using pID3.01 as a template and inserted into a BglII site of pDONR221cav-1BglII. The ORF of CAV-1-GFP was then cloned into pID2.01. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00001826 | |
| Interactor | WBInteraction000502376 | ||
| WBInteraction000502377 | |||
| WBInteraction000502378 | |||
| WBInteraction000518671 | |||
| Reference | WBPaper00027612 | ||
| WBPaper00031064 | |||
| WBPaper00031193 | |||
| WBPaper00032152 | |||
| WBPaper00040428 | |||
| WBPaper00041321 | |||
| WBPaper00044960 |
