WormBase Tree Display for Construct: WBCnstr00000646
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| WBCnstr00000646 | Public_name | pTZ3i | |
|---|---|---|---|
| Summary | [ida-1p::GFP] | ||
| Driven_by_gene | WBGene00002048 | ||
| Gene | WBGene00002048 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | The transcriptional reporter construct pida-1::GFP 2.4 was digested with PstI and KpnI and religated (fill-in). A PCR product from ida-1 cDNA yk27f7 (T3 promoter forward primer 5'-ATTAACCCTCACTAAAGGGA-3'; reverse primer 5'2'-TTATGCATGCGATTGACTTTAGAAG-3') was digested with SphI and inserted into the SphI-cut vector. A BamHI-NheI ida-1 genomic fragment from cosmid B0244 was generated to replace the corresponding BamHI-NheI cDNA fragment, thereby generating the construct pida-1::IDA-1::GFP, which contains a 2443 bp ida-1 promoter upstream of an ida-1 coding region that incorporates introns 3-9 followed at the 3'-end with in-frame GFP coding sequence and terminating with the unc-54 3'-untranslated region. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00000648 | |
| WBTransgene00000649 | |||
| Reference | WBPaper00013484 | ||
| WBPaper00035920 | |||
| WBPaper00043890 |
