WormBase Tree Display for Construct: WBCnstr00000127
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| WBCnstr00000127 | Summary | [unc-54::Q19-GFP; tor-2(D368)(+)] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006598 | ||
| WBGene00006789 | |||
| Gene | WBGene00006598 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | Q19-GFP is a translational fusion of 19 glutamines to GFP. To obtain the D368 mutant form of tor-2, an initial round of PCR was performed to generate an approximately 1 kb cDNA (corresponding to amino acids 1367 of TOR-2) using primers 1 and 3 (5'-GGGAAAAATTCAAGATCAAGAACTCTTTGCATG-3'). In parallel, an ~200 bp fragment (corresponding to amino acids 369412) was amplified with primers 2 and 4 (5'-CATGCAAAGAGTTCTTGATCTTGAATTTTTCCC-3'). The two fragments were then combined, diluted and amplified using primers 1 and 2 to reconstruct the complete cDNA. The mutagenesis of tor-2 was confirmed by DNA sequencing. | ||
| Used_for | Transgene_construct | WBTransgene00000127 | |
| Reference | WBPaper00005722 |
