WormBase Tree Display for Antibody: WBAntibody00002152
expand all nodes | collapse all nodes | view schema
| WBAntibody00002152 | Summary | The egrh-1 ORF encoding amino acids 1-120 was amplified from yk484c12-pOK165.12 and inserted into pMal2c (NEB). Antibodies were raised in rabbits D1919 and D1920 (Open Biosystems) against the MBP::EGRH-1 fusion purified from E. coli BL21 (Ausubel, 1990). EGRH-1 antibodies were purified by sequentially depleting against E. coli extracts expressing maltose-binding protein (MBP) and enriching against purified MBP::EGRH-1 bound to nitrocellulose filters. | ||
|---|---|---|---|---|
| Public_name | [WBPaper00037082]:ant-EGRH-1 | |||
| Gene | WBGene00007772 | |||
| Isolation | Original_publication | WBPaper00037082 | ||
| Location | OK | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | fusion protein of egrh-1 ORF encoding amino acids 1-120 | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr9107 | |||
| Reference | WBPaper00037082 |
