WormBase Tree Display for Expr_pattern: Expr9107
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| Expr9107 | Expression_of | Gene | WBGene00007772 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00007772 | |||
| Expression_data | Anatomy_term | WBbt:0003681 | Certain | |
| WBbt:0004506 | Certain | |||
| WBbt:0004520 | Certain | |||
| WBbt:0005735 | Certain | |||
| WBbt:0005772 | Certain | |||
| WBbt:0005813 | Certain | |||
| WBbt:0005828 | Certain | |||
| WBbt:0006798 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0048471 | ||||
| Subcellular_localization | EGRH-1 was localized to nuclei in all tissues except sperm, where it appeared perinuclear. Importantly, no egrh-1::gfp expression or EGRH-1 antibody staining was observed in oocytes. | |||
| Type | Antibody | |||
| Reporter_gene | ||||
| Pattern | To understand EGRH-1 function in regulating oocyte development, authors examined egrh-1::gfp expression and endogenous EGRH-1 expression using an antibody targeting a unique region at the EGRH-1 N-terminus in dissected adult hermaphrodites. Using both techniques, EGRH-1 expression was observed in the sheath cells and distal tip cells of the somatic gonad, as well as in the intestine and sperm. | |||
| egrh-1::gfp was expressed in embryos through adult stages in most somatic tissues, including the gut, pharynx, body wall muscle, and nervous system. | ||||
| Picture | WBPicture0000007892 | |||
| Remark | Picture: Fig. 5. | |||
| To test if EGRH-1 antibody staining was specific, authors stained egrh-1(tm1736) mutants, which are predicted to encode a truncated protein that would be recognized by our antibody, and egrh-1(tm1736) with egrh-1 expression further reduced by RNAi. Staining was decreased in both cases, indicating that this antibody stain is specific for EGRH-1. | ||||
| Reference | WBPaper00037082 | |||
| Transgene | WBTransgene00008601 | |||
| Antibody_info | WBAntibody00002152 |
