WormBase Tree Display for Antibody: WBAntibody00002135
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| WBAntibody00002135 | Summary | Rat serum EU143 was raised against the predicted C-terminal 353 residues of Ce-titin. Antigens was expressed as GST fusion proteins in E. coli. cDNAs encoding antigenic polypeptides were amplified by RT-PCR using Pfu polymerase (Stratagene) and primers containing added XhoI and EcoRI sites. The primers for EU143 were 5'-GTACGAATTCAAATTCATCTCAGTGACACCAGG-3' and 5'-GATGCTCGAGCTACCGACGAATCGTTCTTCGTTG-3', respectively. Amilified product was cloned into pGEX-6P-1 (Amersham Pharmacia Biotech).T he GST fusion proteins were expressed and purified. Milligram quantities of fusion proteins were supplied to Spring Valley Laboratories (Sykesville, MD) for antiserum production. | ||
|---|---|---|---|---|
| Public_name | [WBPaper00037996]::anti-TTN-1_EU143 | |||
| Other_name | EU143 | |||
| Gene | WBGene00006436 | |||
| Isolation | Original_publication | WBPaper00037996 | ||
| Location | GB | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | the predicted C-terminal 353 residues of CE-titin | ||
| Animal | Rat | |||
| Expr_pattern | Expr9017 | |||
| Reference | WBPaper00036101 | |||
| WBPaper00037996 |
