WormBase Tree Display for Antibody: WBAntibody00002134
expand all nodes | collapse all nodes | view schema
| WBAntibody00002134 | Summary | Guinea pig serum EU145 was raised against the N-terminal 314 residues of the predicted 2.2 MDa isoform of Ce-titin. Antigens was expressed as GST fusion proteins in E. coli. cDNAs encoding antigenic polypeptides were amplified by RT-PCR using Pfu polymerase (Stratagene) and primers containing added XhoI and EcoRI sites. The 5' and 3' primers for EU145 were 5'-GTACGAATTCATGGAGGGCAACGAGAAGAAAGG-3' and 5'-GATGCTCGAGCATTGGGTCAAAGGCAGTGGTCG-3', respectively. Amplified product was cloned into pGEX-6P-1 (Amersham Pharmacia Biotech).T he GST fusion proteins were expressed and purified. Milligram quantities of fusion proteins were supplied to Spring Valley Laboratories (Sykesville, MD) for antiserum production. | ||
|---|---|---|---|---|
| Public_name | [WBPaper0037996]::anti-TTN-1_EU145 | |||
| Other_name | EU145 | |||
| Gene | WBGene00006436 | |||
| Isolation | Original_publication | WBPaper00037996 | ||
| Location | GB | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | N-terminal 314 residues of the predicted 2.2 MDa isoform of Ce-titin | ||
| Animal | Guinea_pig | |||
| Reference | WBPaper00036101 | |||
| WBPaper00037996 |
