WormBase Tree Display for Antibody: WBAntibody00001051
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| WBAntibody00001051 | Summary | Rabbit polyclonal antibody against STA-1 recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [WBPaper00027021]:sta-1 | |||
| Gene | WBGene00013111 | |||
| Isolation | Original_publication | WBPaper00027021 | ||
| Clonality | Polyclonal | |||
| Antigen | Protein | The putative transcriptional regulatory domain (corresponding to amino acids 568 to 703) at the carboxyl-terminus of STA-1 was amplified by PCR from a cDNA construct using forward primer 5' -CGCGGATCCCTGCGTTTCTTCGAGTCC-3' and reverse primer 5' -GGAATTCAGGTAATTGGGGGGACTG-3'. The PCR product was subcloned in-frame into pGEX-4T3 (Pharmacia, Sweden). E. coli strain XL-1 Blue was used for GST fusion protein production. Purified fusion protein was used for immunization of two rabbits (Zymed, CA). | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr3814 | |||
| Interactor | WBInteraction000502299 | |||
| WBInteraction000502300 | ||||
| Reference | WBPaper00027021 |
