WormBase Tree Display for Antibody: WBAntibody00000985
expand all nodes | collapse all nodes | view schema
| WBAntibody00000985 | Summary | Rabbit polyclonal antibody against MEI-1 recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [WBPaper00004129]:mei-1 | |||
| Gene | WBGene00003183 | |||
| Isolation | Original_publication | WBPaper00004129 | ||
| Location | HR | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | Full-length mei-2 cDNA was obtained by RTPCR amplification using 5 and 3 primers containing linkers for insertion into a HIS-tagged fusion vector (QE30) from the QiaExpression kit (Qiagen). Bacterially expressed protein was purified under denaturing conditions (8M urea) by passage through a NiNT Agarose column, using manufacturer's protocol. This purified protein was electrophoresed through an SDS-PAGE gel and excised to immunize rabbits. Anti-MEI-1 serum was affinity purified as above with an amino-terminal portion (residues 13217) obtained by inserting an EcoRV-EcoRI mei-1 cDNA fragment into the GST fusion vector. | ||
| Animal | Rabbit | |||
| Interactor | WBInteraction000502679 | |||
| WBInteraction000502680 | ||||
| WBInteraction000502681 | ||||
| WBInteraction000502682 | ||||
| Reference | WBPaper00004129 | |||
| WBPaper00025044 |
