WormBase Tree Display for Antibody: WBAntibody00000690
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| WBAntibody00000690 | Summary | Polyclonal rabbit antibody against recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [cgc6155]:clk-1_b | |||
| Other_name | 3030 | |||
| Gene | WBGene00000536 | |||
| Isolation | Location | MQ | ||
| Clonality | Polyclonal | |||
| Antigen | Protein | A PCR fragment encoding the full-length CLK-1 protein was cloned into the pGEX-3X expression vector (Amersham Biosciences). A glutathione S-transferase-CLK-1 protein of the expected size (50 kDa) was expressed in BL21(DE3) bacteria (Novagen) and was purified from inclusion bodies. This recombinant protein was injected into rabbits to obtain polyclonal antibodies. The sera of two rabbits (antibodies 2774 and 3030) specifically react to CLK-1. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr2672 | |||
| Reference | WBPaper00006155 | |||
| WBPaper00006240 |
