WormBase Tree Display for Antibody: WBAntibody00000689
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| WBAntibody00000689 | Summary | Polyclonal rabbit antibody against recombinant protein. Antibody 2774 recognizes in vitro translated CLK-1 and an in vitro translated CLK-1 deletion that is potentially encoded by clk-1(qm30). | ||
|---|---|---|---|---|
| Public_name | [cgc6155]:clk-1_a | |||
| Other_name | 2774 | |||
| Gene | WBGene00000536 | |||
| Isolation | Location | MQ | ||
| Clonality | Polyclonal | |||
| Antigen | Protein | A PCR fragment encoding the full-length CLK-1 protein was cloned into the pGEX-3X expression vector (Amersham Biosciences). A glutathione S-transferase-CLK-1 protein of the expected size (50 kDa) was expressed in BL21(DE3) bacteria (Novagen) and was purified from inclusion bodies. This recombinant protein was injected into rabbits to obtain polyclonal antibodies. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr2672 | |||
| Reference | WBPaper00006155 | |||
| WBPaper00006240 |
