WormBase Tree Display for Antibody: WBAntibody00000669
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| WBAntibody00000669 | Summary | Polyclonal rabbit antibody against TAC-1 recombinant protein. Antibodies were affinity purified by using a column coupled to MBP:TAC-1. | ||
|---|---|---|---|---|
| Public_name | [cgc6060]::anti-TAC-1 | |||
| Gene | WBGene00006381 | |||
| Isolation | Location | JA | ||
| Clonality | Polyclonal | |||
| Antigen | Protein | The full-length TAC-1 cDNA was amplified by PCR from EST yk96g12 and was cloned in the pDONR201 vector (Gateway Technology, Invitrogen) to generate entry clone pJA90. A construct for expressing a GST:TAC-1 fusion was generated by recombining pJA90 with pDEST15 to generate pJA91, and another for expressing MBP:TAC-1 was produced by recombining pJA90 with a pmalC2-Gateway vector to generate pJA92. The GST:TAC-1 fusion protein was injected into rabbits for antibody production. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr2683 | |||
| Reference | WBPaper00006060 | |||
| WBPaper00031149 |
