WormBase Tree Display for Antibody: WBAntibody00000387
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| WBAntibody00000387 | Summary | Rabbit polyclonal antibody against recombinant PEF-1 protein. | ||
|---|---|---|---|---|
| Public_name | [cgc4766]:pef-1 | |||
| Gene | WBGene00003969 | |||
| Isolation | Clonality | Polyclonal | ||
| Antigen | Protein | Fusion proteins between the bacteriophage gene 10 protein and the C-terminal 142 amino acids of CePPEF were expressed in Escherichia coli, purified by preparative SDS-PAGE, and used for immunization of rabbits. Fusion proteins between the same CePPEF fragment and maltose-binding protein (MBP) were also produced in E. coli, purified to apparent homogeneity on an amylose resin (New England Biolabs Inc.), covalently cross-linked to Affi-Gel 15 (Bio-Rad), and used to affinity purify the rabbit polyclonal antibodies. | ||
| Animal | Rabbit | |||
| Reference | WBPaper00004766 |
