WormBase Tree Display for Antibody: WBAntibody00000276
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| WBAntibody00000276 | Summary | Polyclonal rat antibodies against recombinant APR-1 protein. Antisera were affinity purified against the recombinant APR-1 fragment immobilized on PVDF membranes (Immobilon). | ||
|---|---|---|---|---|
| Public_name | [cgc3991]:apr-1_a | |||
| Gene | WBGene00000156 | |||
| Isolation | Original_publication | WBPaper00003991 | ||
| Location | AH | |||
| Clonality | Polyclonal | |||
| Antigen | Protein | A 1.3-kb apr-1 cDNA fragment was isolated from a partial cDNA clone by PCR amplification with the primers 5 -TTTGGATCCACGGAGGATCAAGCGCAAATG-3 and 5 -TTTGGATCCGAACAGGCAGTCTTGACTTTCC-3 and cloned into the BamHI site of the Escherichia coli expression vector pDS6 to produce an APR-1 fragment consisting of amino acids 551 to 983 with an amino-terminal His6 tag. The His6::APR-1 fusion protein was produced in the E. coli strain M15, purified on NTA agarose according to the manufacturer's instructions (Qiagen), and used to immunize rats and rabbits. | ||
| Animal | Rat | |||
| Expr_pattern | Expr1046 | |||
| Reference | WBPaper00003991 |
