WormBase Tree Display for Antibody: WBAntibody00000197
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| WBAntibody00000197 | Summary | Polyclonal rabbit anti-dynamin antibodies against recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [cgc3275]:dyn-1 | |||
| Gene | WBGene00001130 | |||
| Isolation | Original_publication | WBPaper00003275 | ||
| Location | NM | |||
| TY | ||||
| ZA | ||||
| Clonality | Polyclonal | |||
| Antigen | Protein | C-terminal half of C. elegans dynamin was expressed in E. coli with the bacterial expression vector pQE30 (Qiagen, Valencia, CA). This vector adds six histidines, which was used to purify the recombinant protein by Ni-affinity chromatography. Rabbit antisera were generated by Cocalico Biologicals (Reamstown, PA) and then blot purified with a dynamin protein fragment. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr512 | |||
| Expr2307 | ||||
| Interactor | WBInteraction000555225 | |||
| WBInteraction000555226 | ||||
| Reference | WBPaper00006494 | |||
| WBPaper00005664 | ||||
| WBPaper00034638 | ||||
| WBPaper00003275 |
