WormBase Tree Display for Antibody: WBAntibody00000190
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| WBAntibody00000190 | Summary | Rabbit polyclonal antibody against PGL-1 recombinant protein. | ||
|---|---|---|---|---|
| Public_name | [cgc3186]::anti-PGL-1 | |||
| Other_name | OIC4D | |||
| Gene | WBGene00003992 | |||
| Isolation | Original_publication | WBPaper00003186 | ||
| Location (14) | ||||
| Clonality | Polyclonal | |||
| Antigen | Protein | The PGL-1 expression vector was constructed by cloning a 1071 bp HincII fragment of pgl-1 cDNA, which encodes the central 357 amino acids of PGL-1 (Asn-195 to Val-551), into the expression vector pQE-32 (Qiagen). A 6 His-tagged fusion protein of the expected size ( 45 kDa) was expressed in E. coli and purified using a Ni-NTA agarose column (Qiagen). The purified fusion protein was injected into rabbits. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr2999 | |||
| Interactor (12) | ||||
| Reference (67) |
