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WormBase Tree Display for Expr_pattern: Expr970

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Name Class

Expr970Expression_ofGeneWBGene00003623
Reflects_endogenous_expression_ofWBGene00003623
HomolHomol_homolF11C1:Expr
Expression_dataLife_stageWBls:0000022
WBls:0000015
WBls:0000014
WBls:0000021
Anatomy_term (40)
GO_termGO:0005634
Subcellular_localizationnuclei
TypeReporter_gene
PatternIn comma to 1.5-fold stage embryos, the nhr-25::GFP are expressed in the V cells, P cells and hyp7. Expression also observed in the head and tail hypodermal cells of embryos. The earliest expression is at ~250-300 min post fertilization. atEx32 and atEx35 L1 animals exhibited consistent reporter expression in the hyp7 and P cell nuclei. The P cell expression was very strong at hatching. GFP expression in the P cell and hyp7 decreased during mid-L1, but frequently increased late L1. At hatching no expression in observed in the V cells. In mid L1 the V cells divided and the anterior daughters begin to express GFP as they join the syncytium. Strong expression is also seen in the head and tail hypodermal nuclei of L1 larva. Expression in the hypodermal nuclei of older larva stages is similar to that in the L1. GFP expression decreases markedly in adults. GFP is also observed in other ectodermal cells. In L1, expression is observed in the G2(excretory pore) cell and in a cell tentatively identified as the W neuroblast. In older larva, expression continues in G2 but disappears in the W lineage once the cells divides to generate neurons. Beginning in L2, GFP is expressed in the region around the rectum. Expression is also occasionally observed in the anterior pharynx, in the nuclei with positions consistent with those of the pharyngeal epithelial cells.
RemarkData observed from, atIs13, atEx32 and atEx35.
ReferenceWBPaper00004124
TransgeneWBTransgene00000074