WormBase Tree Display for Expr_pattern: Expr9661
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| Expr9661 | Expression_of | Gene | WBGene00003107 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003107 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| Anatomy_term (12) | |||
| Type | In_situ | Single molecule fluorescence in situ hybridization (smFISH) was performed based on protocols described previously (Raj et al., 2008) and at www.singlemoleculefish.com. For mab-10 probes conjugated to Cy5, a probe dilution of 1/10,000 from the original stock solution was used. Probes for mab-10 were designed following the guidelines specified at www.singlemoleculefish.com and purchased from Biosearch Technologies (Novato, CA). | |
| Pattern | We observed during the L1 stage mab-10 transcripts in the anterior and posterior bulbs of the pharynx as well as a small number of transcripts throughout the hypoderm. L2-stage animals showed increased expression of mab-10 mRNA in hyp7 and the rectal epithelium in addition to the nerve ring and the ventral nerve cord, where several cells contained one or two transcripts. During the early L3 stage, mab-10 was weakly expressed in hyp7 but showed high expression in the vulval and uterine precursor cells. mab-10 mRNA was also present within the distal tip cells and a pair of bilaterally symmetric cells that we believe to be the CAN neurons, based on their position and neuronal nuclear morphology. By the late L3 stage, mab-10 expression was almost absent from hyp7 but was high in the seam cells, distal tip cells and developing vulva. mab-10 transcript was also detected in the gonadal sheath cells. During the L4 stage, mab-10 transcripts dramatically increased in abundance throughout the pharynx, hypoderm and somatic gonad, including the distal tip cells. | ||
| Reference | WBPaper00040121 |
