WormBase Tree Display for Expr_pattern: Expr9287
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| Expr9287 | Expression_of | Gene | WBGene00010813 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00010813 | |||
| Expression_data | Anatomy_term | WBbt:0003679 | Partial | |
| WBbt:0003826 | Certain | |||
| WBbt:0003827 | Certain | |||
| WBbt:0003903 | Certain | |||
| WBbt:0003904 | Certain | |||
| GO_term | GO:0030424 | |||
| GO:0043025 | ||||
| GO:0048786 | ||||
| GO:0035869 | ||||
| Subcellular_localization | A functional PITP-1::Venus fusion protein was specifically expressed in ASER to determine the subcellular localization of PITP-1. PITP-1::Venus was mainly localized in a punctate pattern along the axon and in the cell body, and colocalized with the synaptic vesicle marker mCherry::RAB-3 in the axon, indicating that PITP-1::Venus is localized to presynaptic sites. A few inconsistent puncta were often observed along the dendrites, but this might be mislocalization because of over-expression of the transgene. In addition to the presynaptic localizations, PITP-1::Venus was observed consistently at the ciliary transition zone. | |||
| Type | Reporter_gene | [Ppitp-1::venus] [PITP-1::Venus] transcriptional and translational fusions. The pitp-1 cDNA was obtained by PCR from C. elegans cDNA generated by reverse-transcription reaction using total RNA as a template and SuperScriptIII kit (Invitrogen) and ligated between KpnI and EcoRV in the pPD-DEST vector (pDEST- pitp-1). For C-terminal Venus tagging, venus::unc-54 3' UTR was PCR-amplified from pDEST-venus vector and fused to the C terminus of pitp-1 cDNA, amplified as described above by PCR. The fused PCR fragment was ligated between KpnI and SpeI in the pPD-DEST vector (pDEST-pitp-1::venus). | ||
| Pattern | Ppitp-1::venus transcriptional reporter displayed weak fluorescence in many, but not all, neurons throughout the nervous system. Coexpression with mCherry driven by various promoters confirmed that pitp-1 is expressed in sensory neurons ASE and AWC. Expression in ASH by using this reporter was not detected. The widespread but specific expression pattern in the nervous tissue is analogous to the expression pattern of RdgB in the Drosophila brain. | |||
| Reference | WBPaper00038331 | |||
| Transgene | WBTransgene00008884 |
