WormBase Tree Display for Expr_pattern: Expr9180
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| Expr9180 | Expression_of | Gene | WBGene00002275 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002275 | ||
| Expression_data | GO_term | GO:0005643 | |
| GO:0031965 | |||
| Subcellular_localization | By immunofluorescence microscopy, these antibodies exclusively stained the nuclear membrane of wild-type C. elegans embryos, whereas they did not produce specific signal in lem-2 mutant embryos. Higher magnification of nuclei shows that LEM-2 apparently coats the entire nuclear membrane, with areas of slightly less signal at sites occupied by nuclear pore complexes (NPCs). | ||
| Type | Antibody | Rabbit polyclonal antibodies directed against the amino terminus of LEM-2 protein | |
| Western | The specificity of the antibodies was confirmed by western blotting, which detects a strong band at the expected size of 55 kDa in wild-type C. elegans embryos. | ||
| Reference | WBPaper00037942 | ||
| Antibody_info | WBAntibody00002216 |
