WormBase Tree Display for Expr_pattern: Expr8981
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| Expr8981 | Expression_of | Gene | WBGene00001819 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001819 | |||
| Expression_data | Anatomy_term | WBbt:0005772 | Certain | |
| GO_term | GO:0005765 | |||
| GO:0016020 | ||||
| Subcellular_localization | Double labeling of intestinal cells with acridine orange, a dye for acidic compartment staining. As a result, HAF-4::GFP-positive and acridine orange-positive granules turned out to be mutually exclusive, and the pattern was same in case of HAF-9:: GFP-positive granules. A similar exclusive pattern was observed when LysoTracker Red, another dye for monitoring the acidification of subcellular compartments, was used in place of acridine orange. | |||
| In intestinal cells of the late larval and adult stages, HAF-4::GFP and HAF-9::GFP seemed, under confocal microscopy, to be distributed on the surface of intestinal granules of some sort. These results indicate that HAF-4 and HAF-9 are present on the membranes of some intestinal granules. The HAF-4::GFP-positive and HAF-9::GFP-positive granules were relatively large (>2 um average diameter) and accounted for the majority of distinguishable intestinal granules. | ||||
| Type | Reporter_gene | |||
| Pattern | GFP expression was observed at the larval and adult stages exclusively in all of the 20 intestinal cells of which a whole intestine consists. At the embryonic stage, no expression was found. | |||
| Picture | WBPicture0000008867 | |||
| WBPicture0000008870 | ||||
| Remark | Picture: Fig 2, Fig 3, Fig S2A. | |||
| Reference | WBPaper00033097 | |||
| Transgene | WBTransgene00030841 |
