WormBase Tree Display for Expr_pattern: Expr7891
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| Expr7891 | Expression_of | Gene | WBGene00010470 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00010470 | |||
| Expression_data | Anatomy_term | WBbt:0003681 | Certain | |
| WBbt:0003721 | Certain | |||
| WBbt:0003724 | Certain | |||
| WBbt:0003737 | Certain | |||
| WBbt:0005772 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0005764 | ||||
| Subcellular_localization | It was concentrated in small punctate structures within these cells. The size and distribution of these structures were similar to those of lysosomes and of CDR-1-eGFP. It has been shown that when a nonfusion form of eGFP is expressed in intestinal cells, it accumulates in the cytoplasm, not in vesicles. The transgenic nematodes were fed rhodamine B isothiocyanate (RITC)-labeled dextran, which labels intestinal cell lysosomes, to confirm that CDR-4 was targeted to the lysosomes. In the double-labeling experiment, both eGFP and rhodamine colocalized to the same structures, confirming that CDR-4 is targeted to intestinal cell lysosomes. | |||
| Type | Reporter_gene | |||
| Pattern | The CDR-4-eGFP fusion protein was expressed predominately in the intestinal cells of the nematodes. | |||
| Picture | WBPicture0000010612 | |||
| WBPicture0000010613 | ||||
| Remark | Picture: Figure 6. | |||
| Reference | WBPaper00031378 | |||
| Transgene | WBTransgene00030177 |
