WormBase Tree Display for Expr_pattern: Expr7891
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Expr7891 | Expression_of | Gene | WBGene00010470 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00010470 | ||
Expression_data | Anatomy_term (5) | ||
GO_term | GO:0005737 | ||
GO:0005764 | |||
Subcellular_localization | It was concentrated in small punctate structures within these cells. The size and distribution of these structures were similar to those of lysosomes and of CDR-1-eGFP. It has been shown that when a nonfusion form of eGFP is expressed in intestinal cells, it accumulates in the cytoplasm, not in vesicles. The transgenic nematodes were fed rhodamine B isothiocyanate (RITC)-labeled dextran, which labels intestinal cell lysosomes, to confirm that CDR-4 was targeted to the lysosomes. In the double-labeling experiment, both eGFP and rhodamine colocalized to the same structures, confirming that CDR-4 is targeted to intestinal cell lysosomes. | ||
Type | Reporter_gene | ||
Pattern | The CDR-4-eGFP fusion protein was expressed predominately in the intestinal cells of the nematodes. | ||
Picture | WBPicture0000010612 | ||
WBPicture0000010613 | |||
Remark | Picture: Figure 6. | ||
Reference | WBPaper00031378 | ||
Transgene | WBTransgene00030177 |