WormBase Tree Display for Expr_pattern: Expr713
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| Expr713 | Expression_of | Gene | WBGene00004078 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004078 | ||
| Expression_data | Life_stage | WBls:0000038 | |
| WBls:0000041 | |||
| WBls:0000005 | |||
| Anatomy_term | WBbt:0004015 | ||
| WBbt:0005175 | |||
| WBbt:0006773 | |||
| WBbt:0006873 | |||
| WBbt:0006874 | |||
| WBbt:0006875 | |||
| WBbt:0006876 | |||
| GO_term | GO:0005737 | ||
| Subcellular_localization | POS-1 is cytoplasmic. | ||
| Type | Antibody | Antisera raised against a fusion protein containing N-terminal region of POS-1 and against a synthetic peptides corresponding to regions that are divergent in sequence from MEX-1 protein. | |
| Pattern | First detected at low levels in some 1-cell embryos. At the 2-cell stage higher level of expression detected predominantly in germline blastomere P1. Distribution of POS-1 protein is similar to distribution of mRNA after 2-cell stage when P1 divides. POS-1 protein is initially present at equal levels in both P1 daughters but persists at higher level only in germline daughter P2. After the division of the final germline blastomere P4, POS-1 is not detected in any cell. POS-1 shows strong, specific staining of granules within the cytoplasm of germline blastomeres. In double labeling experiments with an Ab specific for P granules determined that POS-1 is associated with cytoplasmic and perinuclear P granules. Although P granules is present in germline at all stages of embryonic and postembryonic development, POS-1 staining is only seen in P granules in germline blastomeres, P1, P2, P3 and P4. POS-1 gene is expressed maternally, POS-1 is cytoplasmic, P granule associated protein is present predominantly in germline blastomeres during early embryogenesis. | ||
| Picture | WBPicture0000008000 | ||
| Remark | This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). | ||
| Reference | WBPaper00003382 | ||
| Antibody_info | WBAntibody00000207 | ||
| WBAntibody00000208 |
