WormBase Tree Display for Expr_pattern: Expr711
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| Expr711 | Expression_of | Gene | WBGene00004042 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004042 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| Anatomy_term | WBbt:0006797 | ||
| GO_term | GO:0005737 | ||
| GO:0005813 | |||
| Subcellular_localization | located at centrosomes after pronuclear meeting in post-meiotic 1-cell embryos. PLK-1 remains associated with centrosomes as the embryo enters prophase of mitosis and this localization persists through late anaphase. PLK-1 staining in mature, unfertilized oocytes is diffuse and cytoplasmic. | ||
| Type | Antibody | PLK-1 antibody was generated against a peptide corresponding to the amino-terminal 20 amino acids of the PLK-1 by conjugation to keyhole limpet hemocyanin and injection into rabbit. | |
| Pattern | First detected at centrosomes after pronuclear meeting in post-meiotic 1-cell embryos. PLK-1 remains associated with centrosomes as the embryo enters prophase of mitosis and this localization persists through late anaphase. PLK-1 also detected in chromosome-specific localization of PLK-1 diminishes during anaphase and is not observed during telophase. PLK-1 detected at the region of spindle overlap during telophase (coincident with alpha-tubulin staining). At the time this appears as two discrete points suggesting it was part of a ring-like structure. PLK-1 is detected in newly fertilized embryos. PLK-1 staining in mature, unfertilized oocytes is diffuse and cytoplasmic. In new fertilized embryos, PLK-1 staining is coincident with six maternal bivalent pre-metaphase chromosome before their segregation. As fertilized embryos progressed through anaphase of meiosis, PLK-1 was again coincident with the dividing chromatin but has also redistributed and was found in the areas between the dividing chromatin. | ||
| Remark | This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). | ||
| Reference | WBPaper00004215 | ||
| Antibody_info | WBAntibody00000294 |
