WormBase Tree Display for Expr_pattern: Expr697
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| Expr697 | Expression_of | Gene | WBGene00003229 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003229 | ||
| Expression_data | Life_stage | WBls:0000005 | |
| Anatomy_term | WBbt:0004015 | ||
| WBbt:0004421 | |||
| WBbt:0005906 | |||
| WBbt:0005994 | |||
| WBbt:0006873 | |||
| WBbt:0006874 | |||
| WBbt:0006875 | |||
| WBbt:0006876 | |||
| Type | Antibody | Generated in mice from bacterially expressed full-length MEX-3 or the COOH-terminal 187 amino acids of MEX-3. Monoclonal and polyclonal antisera gave identical staining patterns. | |
| Pattern | MEX-3 is present as a cytoplasmic protein. MEX-3 protein is first detected in oocytes shortly after cellularlization and the amount of protein appears to increase in oocytes as they mature. After fertilization, MEX-3 protein is evenly distributed throughout the cytoplasm of the 1 cell stage embryo. By the end of the 2-cell stage, MEX-3 protein appears more abundant in the AB blastomere than in P1 blastomere. In the 4-cell stage, the protein appears more abundant in the AB daughters than the P1 daughters. After 4-cell stage MEX-3 protein disappears from the embryo. In addition to general cytoplasmic staining, each of the MEX-3 antisera specifically stained distinct particles in P1 and in the P1 descendants P2, P3 and P4. Simultaneous staining with an anti-MEX-3 antibody and anti-P granule antibody, shows the particles stained by the MEX-3 antisera coincide with P granules in the blastomeres P1, P2, P3 and P4. However, the MEX-3 antisera do not stain P granules in the gonad or P granules in the germ cells of late stage embryos. | ||
| Picture | WBPicture0000007969 | ||
| Remark | This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). | ||
| Reference | WBPaper00002922 | ||
| Antibody_info | WBAntibody00000153 | ||
| WBAntibody00000154 |
