WormBase Tree Display for Expr_pattern: Expr679
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| Expr679 | Expression_of | Gene | WBGene00003920 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003920 | ||
| Expression_data | Life_stage | WBls:0000003 | |
| WBls:0000041 | |||
| Anatomy_term | WBbt:0005175 | ||
| WBbt:0006797 | |||
| Type | In_situ | hybridization ftt-1 cDNA clone pSM74 was linearized with EcoRI and anti-sense probes were generated in vitro using T7 primer and multiple cycles of primer extension in the presence of digoxingenin-dUTP. ftt-1 sense probes were generated from pSM74 using XhoI to first linearize clone before initiating primer extension reaction with T3 primer. | |
| Pattern | Using probe that hybridizes to all three transcripts. In one cell embryo, high levels of ftt-1 transcripts found uniformly throughout the cell. At 1-fold embryonic stage, transcript level decreases as embryo develops until no longer detected at the 1.5-fold stage. ftt-1 transcripts detected throughout entire gonad without any obvious subcellular localisation patterns. Most abundant in distal mitotic region and their concentration gradually decreases throughout the syncytial pachytene region and into maturing oocytes. No expression seen in the distal tip cell. | ||
| Picture | WBPicture0000011638 | ||
| WBPicture0000011641 | |||
| WBPicture0000011644 | |||
| Remark | This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). | ||
| see Expr680 for Northern analysis result. | |||
| Reference | WBPaper00002793 |
