WormBase Tree Display for Expr_pattern: Expr4285
expand all nodes | collapse all nodes | view schema
| Expr4285 | Expression_of | Gene | WBGene00006543 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006543 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0003721 | Certain | ||
| WBbt:0003737 | Certain | |||
| WBbt:0003739 | Certain | |||
| WBbt:0003740 | Certain | |||
| WBbt:0005439 | Certain | |||
| WBbt:0005813 | Certain | |||
| GO_term | GO:0005634 | |||
| Subcellular_localization | This full-length fusion protein is nuclear localized. | |||
| Type | Reporter_gene | |||
| Pattern | In transgenic embryos, this larger tbx-2::gfp reporter was expressed in a dynamic pattern, including in a subset of pharyngeal precursors in the premorphogenetic embryo, as well as body wall muscle and pharyngeal neurons. tbx-2::gfp expression initiated in 2 anterior cells in approximately 100 cell embryos and increased to 12 cells by approximately the 200 cell stage. The increasing number of GFP expressing cells was not due to cell divisions; rather tbx-2::gfp expression appeared to initiate asynchronously in individual cells. Expression in these cells was transient and was undetectable by the bean stage. Prior to the bean stage, tbx-2::gfp expression was also observed in body wall muscles, and later, in 2- to 3-fold embryos, expression was observed in a number of pharyngeal neurons. Expression in both of these tissues continued in larvae. | |||
| Remark | Embryos at the ~200 cell stage contain 24 clonally committed pharyngeal precursors located in the anterior of the embryo (13 ABa-derived and 11 MS-derived), and the location of the 12 tbx-2::gfp expressing cells at this stage suggests that they are included among these precursors. To determine if these early tbx-2::gfp expressing cells were indeed pharyngeal precursors and to characterize their lineal origin, athors examined expression of a tbx-2::gfp promoter fusion containing the same tbx-2 5'-flanking sequences characterized above fused to gfp just downstream of the tbx-2 translation initiation codon (pOK206.30). Previous studies demonstrated that such promoter fusions can produce a longer lived GFP signal than full-length protein fusions. This tbx-2::gfp promoter fusion produced cytoplasmic GFP first detected in premorphogenetic embryos in the same pattern as full-length fusion protein, but GFP perdured in the pharynx until near hatching. GFP expression was observed in 3-fold embryos and early larvae in a reproducible subset of pharyngeal muscles, with occasional expression in body wall muscles and head neurons. GFP was observed predominantly in pharyngeal muscle types derived solely from ABa or from mixed lineages, and the number of these GFP-positive cells was generally consistent with those containing ABa-derived cells. However, exceptions to this generalization were found. Most notably, GFP was reproducibly observed in one MS-derived m7 muscle and all three m4 muscles (of which 2 contain only MS-derived cells). Taken together, these results strongly suggest that tbx-2::gfp expression in premorphogenetic embryos is limited to pharyngeal precursors, and most of these are ABa-derived, although expression is also likely in a small number of MS-derived pharyngeal precursors. | |||
| Reference | WBPaper00027631 | |||
| Transgene | WBTransgene00029096 |
