WormBase Tree Display for Expr_pattern: Expr3802
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| Expr3802 | Expression_of | Gene | WBGene00015083 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00015083 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| GO_term | GO:0005886 | |||
| Subcellular_localization | EGG-1:GFP (pie-1 promoter, N-terminal GFP fusion) appear to localize to the plasma membrane of developing oocytes. The GFP fusion proteins display a swiss-cheese appearance when viewed at high magnification using 3D-deconvolution microscopy. To test for cell surface localization of EGG-1, a live-cell staining assay was developed in which anti-GFP sera was injected into the uterus of hermaphrodites. Anti-GFP staining was observed on the surface of oocytes in the proximal gonad arms and uterus of animals expressing the EGG-1:GFP fusion (C-terminal fusion, GFP on surface). Further, this staining colocalized with GFP fluorescence. No oocyte surface staining was seen with the anti-GFP sera injected into the uterus of hermaphrodites expressing the GFP:EGG-1 (N-terminal fusion, GFP in the cytoplasm). These experiments demonstrate that EGG-1:GFP is on the egg plasma membrane. | |||
| Type | Reporter_gene | |||
| Pattern | EGG-1:GFP driven by its own promoter is restricted to the adult germ line. | |||
| Picture | WBPicture0000009818 | |||
| Reference | WBPaper00026987 | |||
| Transgene | WBTransgene00004934 |
