WormBase Tree Display for Expr_pattern: Expr3683

Expr3683 Expression_of: Gene: WBGene00006943
    Reflects_endogenous_expression_of: WBGene00006943
  Expression_data: GO_term: GO:0005634
      GO:0005737
      GO:0005938
  Subcellular_localization: In the progeny of many cells, the nuclear level of WRM-1::GFP and GFP::LIT-1 was higher in the posterior daughters than in the anterior ones; this observation included hypodermal cells (T, V, and P cells), neuroblasts (QL and QR), and mesodermal cells (Mdr and Mvr). The asymmetric nuclear localization of LIT-1 is also reported in embryos. Weak punctate fluorescence was often detected at the anterior side of some cells before their division, for both GFP::LIT-1 and WRM-1::GFP. The asymmetric localization of GFP::LIT-1 and WRM-1::GFP was more clearly observed in seam cells (Vn.p), especially in the V5.p cell at the end of the L1 stage, when the expression of these genes was higher. Punctate fluorescence was clearly visible near the cell membrane in the anterior half of the cells. The asymmetric cortical localization was also observed during the mitosis of the V5.p cell. No asymmetry in the cytoplasmic localization of WRM-1 or LIT-1 was observed before or during cell division. At telophase, their localization near the anterior cortex and in the newly formed posterior nucleus was observed. Although WRM-1::GFP was nearly undetectable in the anterior nucleus after the V5.p division, it was clearly detected in the anterior nucleus at telophase, suggesting that the WRM-1 protein is exported out of the anterior nucleus during late telophase. These results indicate that WRM-1 and LIT-1 are asymmetrically localized to the anterior cortex before and during division and to the posterior nucleus after the division of many cells during post-embryonic development.
  Type: Reporter_gene
  Picture: WBPicture0000008495
    WBPicture0000008498
  Reference: WBPaper00026737
  Transgene: WBTransgene00001434