WormBase Tree Display for Expr_pattern: Expr3661
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| Expr3661 | Expression_of | Gene | WBGene00012156 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00012156 | ||
| Expression_data | GO_term | GO:0005874 | |
| Subcellular_localization | Small dots of fluorescence were observed moving away from centrosomes. In addition to the moving fluorescent dots, a strong fluorescence signal was observed throughout the centrosomal region, likely indicating a large population of growing microtubules very near the centrosome. To test whether EBP-2 localization to centrosomes was microtubule dependent, EBP-2::GFP fluorescence was examined after RNAi of alpha- and beta-tubulin. Two classes of embryos were observed : those that contained a very low albeit detectable EBP-2::GFP signal at the centrosome (5/12), and those that had no obvious foci of EBP-2::GFP fluorescence (7/12). In all cases where EBP-2 was detected, short EBP-2::GFP tracks quickly disappeared less than one micron from the centrosome. Therefore, the EBP-2::GFP signal is dependent on growing microtubules. | ||
| Type | Reporter_gene | ||
| Picture | WBPicture0000009978 | ||
| WBPicture0000009979 | |||
| Reference | WBPaper00026660 | ||
| Transgene | WBTransgene00000389 |
