WormBase Tree Display for Expr_pattern: Expr3280
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| Expr3280 | Expression_of | Gene | WBGene00000845 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000845 | ||
| Expression_data | GO_term | GO:0005886 | |
| GO:0005783 | |||
| GO:0005794 | |||
| GO:0031410 | |||
| Subcellular_localization | In living worms, CUP-4::GFP localizes primarily to vesicles scattered around the cytoplasm. While some of the CUP-4::GFP localized around the nucleus, presumably in the endoplasmic reticulum (ER) membrane, the general pattern of localization of CUP-4::GFP was quite distinct from that of cytochrome b5, a marker for smooth ER, and from TRAM, a marker for the rough ER. To ascertain whether CUP-4 exits the ER, the transgene expressing CUP-4::GFP was crossed into a cup-5(ar465) background in which endocytosed material is not degraded and accumulates in large vacuoles. CUP-4::GFP, but neither of the ER markers, localized to the membrane of the large vacuoles. These results are consistent with CUP-4::GFP localizing at steady state to the Golgi apparatus or to the plasma membrane/endosomes. The residual ER accumulation of CUP-4::GFP in the cup-4(+) background is eliminated in a cup-4(ok837) background, suggesting that slight overexpression increases retention in the ER. | ||
| Type | Reporter_gene | ||
| Picture | WBPicture0000009798 | ||
| WBPicture0000009800 | |||
| Reference | WBPaper00025238 | ||
| Transgene | WBTransgene00028501 |
