WormBase Tree Display for Expr_pattern: Expr3122
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| Expr3122 | Expression_of | Gene | WBGene00000010 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000010 | |||
| Homol | Homol_homol | Y53H1C:Expr | ||
| Expression_data | Anatomy_term | WBbt:0003679 | Partial | |
| WBbt:0005813 | Partial | |||
| Type | Reporter_gene | |||
| Pattern | The transgenic progeny containing this extrachromosomal aat-9::gfp fusion construct showed a strong specific signal in the neuronal body, the position (lateral to the anterior pharyngeal bulb) and morphology of which resemble those of the specifically stained neuron detected by immunofluorescence. In addition, a prominent signal was present in a symmetrical pair of neurons also located in the vicinity of the anterior pharyngeal bulb. With processes running toward the tip of the animals extreme anterior, this pair of neurons displays a typical feature of chemosensory neurons exposed to the environment through specialized structures in the cuticle. A less prominent signal was observed in several additional neuronal bodies localized between the two pharyngeal bulbs. In addition, a signal was visible in the body wall muscles around the worm mouth. The signal detected in the posterior region was considered autofluorescence (as visible in other animals). Immunostaining of AAT-9 in whole wild type worms showed a weak but reproducible signal in at least one anterior neuronal body and its process. A strong, non-homogenous, presumably non-specific staining was also observed in the head of the worm (as with other antibodies). The extended localization observed with the transcriptional construct suggests that AAT-9 is expressed in several additional neurons and muscle cells, possibly at a lower level than in the neuron labeled by immunofluorescence. | |||
| Picture | WBPicture0000008266 | |||
| Reference | WBPaper00024381 | |||
| Transgene | WBTransgene00028373 |
