WormBase Tree Display for Expr_pattern: Expr3120

Expr3120 Expression_of: Gene: WBGene00004875
    Reflects_endogenous_expression_of: WBGene00004875
  Expression_data: Life_stage: WBls:0000024
      WBls:0000038
      WBls:0000027
      WBls:0000035
      WBls:0000041
      WBls:0000015
      WBls:0000021
      WBls:0000010
      WBls:0000013
    Anatomy_term: WBbt:0003679 Partial
      WBbt:0005772 Certain
  Type: Reporter_gene
  Pattern: Substantial fluorescence was noted in the 20 intestinal cells of adult and larval stages. During the embryonic development GFP signals were first seen in eggs that are situated in the uterus close to the vulva, representing embryos at the time of gastrulation, approximately 3 h after fertilisation. The AdoMetDC promoter drives GFP expression in the anterior part of the nervous system and in two neurons located in the posterior midbody. Remarkably, males exhibit a slightly different neuronal GFP expression pattern. Besides the neurons in the pharyngeal region and in the posterior midbody, six fluorescent ray neurons were detected.
  Picture: WBPicture0000011828
  Remark: The constructs pPD95.77CeODC(2047), pPD95.77CeAdoMet(3720) and pPD95.77CeSPDS(1687) were generated using the same sense primers as above but the anti sense primers 5'-GGA TCC AAT GTC ACG GCA CAT TTG GAG-3' (ODC-AS1), 5'-CCC GGG AAA GTT GGT GGC AGA CGT GGC-39 (AdoMet-AS1) GGA TCC GTT CAT GGC TTT TCG GAG (SPDS-AS1), respectively, that are located within the first 50 bp of the open reading frames. The constructs pPD95.77CeSPDS(1687), pPD95.77CeAdoMet(3720) and pPD95.77CeODC(2047) exhibited the same expression pattern as pPD95.77CeSPDS-1, pPD95.77CeAdoMet-1 or pPD95.77CeODC-1, indicating that the regions downstream of the translational start site do not contain promoter elements that affect the expression pattern of the three genes.
  Reference: WBPaper00024333
  Transgene: WBTransgene00028371