WormBase Tree Display for Expr_pattern: Expr3106
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| Expr3106 | Expression_of | Gene | WBGene00006890 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006890 | ||
| Homol | Homol_homol | K07E3:Expr | |
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000041 | |||
| WBls:0000015 | |||
| WBls:0000021 | |||
| WBls:0000010 | |||
| WBls:0000013 | |||
| Anatomy_term (17) | |||
| Type | Reporter_gene | ||
| Pattern | A VEM-1::GFP translational reporter construct by inserting GFP in frame before the stop codon in the VEM-1 protein sequence. Transgenic animals expressing either the translational or the transcriptional vem-1 reporter displayed the same labeling pattern. Importantly, animals harboring the translational reporter confirm that VEM-1 is expressed by a subset of nerve ring neurons and the AVG pioneer neurons, each of which extend GFP-labeled axons into the VNC. | ||
| Transcriptional fusion: In transgenic embryos, vem-1::GFP expression was first detected at the beginning of gastrulation in a small number of unidentified cells. During later embryonic stages, promoter activity was evident in a subset of putative anterior head neurons. Strong GFP labeling was also detectable in what is likely to be the AVG pioneer neuron and in the assembling VNC as early as the 1.5-fold stage. These findings suggest that vem-1 is expressed by a subset of early developing neurons that extend axons into the right VNC. By the L1 stage, vem-1::GFP activity was clearly detectable in several bilaterally symmetric neurons, including the following: CEPDL/R, RMDVL/R, RIVL/R, AVAL/R, RMDL/R, and RMDDL/R and in the AVG. At the L4 stage, vem-1::GFP continues to be expressed by a subpopulation of nerve ring neurons and by AVG. The presence of GFP along the dorsalmost axon of the VNC is consistent with the AVG neuron expressing vem-1::GFP. Similarly, the GFP labeling observed in a small subset of more ventrally positioned right VNC-associated axons identifies AV (anterior ventral) interneurons as additional sites of vem-1::GFP transgene expression. Consistent with vem-1::GFP expression being restricted to a subset of longitudinally projecting interneurons, axons extending from circumferentially projecting motor neurons were not labeled. Notably, the labeling of the intestine and pharyngeal muscles was highly mosaic, because it was only detected in a small subset of transgenic animals. In contrast, labeled neurons were observed in each transgenic animal harboring the vem-1::GFP transcriptional reporter construct. | |||
| Picture | WBPicture0000011120 | ||
| Reference | WBPaper00024484 | ||
| Transgene | WBTransgene00002007 |
