WormBase Tree Display for Expr_pattern: Expr2692

Expr2692 Expression_of: Gene: WBGene00003150
    Reflects_endogenous_expression_of: WBGene00003150
  Expression_data: GO_term: GO:0005694
      GO:0005813
      GO:0043186
      GO:0005938
  Subcellular_localization: GFP:MBK-2 foci was detected in 0/9 embryos undergoing metaphase or anaphase of meiosis I, in 3/4 embryos in telophase of meiosis I or prophase of meiosis II, and in 18/18 embryos in metaphase or anaphase of meiosis II. After pronuclear formation, the foci disappeared quickly in a posterior-to-anterior wave. GFP:Histone H2B fusion was used to precisely stage embryos expressing GFP:MBK-2.
    GFP:MBK-2 was found predominantly at the cell periphery (cortex) in a uniform pattern. In later zygotes, GFP:MBK-2 distribution changed abruptly from uniform to punctate, with GFP:MBK-2 apparently coalescing into many discrete cortical foci. By mitosis, GFP:MBK-2 was found predominantly on centrosomes and chromosomes, in a pattern that persisted in all blastomeres up to at least the eight-cell stage. In the germline blastomeres P2, P3, and P4, GFP:MBK-2 also appeared to associate with P granules.
    Seventy-eight percent of mat-1(RNAi) embryos (n=292) maintained GFP:MBK-2 uniformly distributed at the cortex, as it is in oocytes and newly fertilized zygotes. The remainder typically had fewer foci than wild-type. mat-1 encodes CDC27, an APC subunit required for the metaphase-to-anaphase transition. Thus, redistribution of MBK-2 into cortical foci occurs just prior to the second meiotic division, and depends on progression past metaphase of meiosis I.
  Type: Reporter_gene
  Picture: WBPicture0000009921
  Reference: WBPaper00006085
  Transgene: WBTransgene00028130