WormBase Tree Display for Expr_pattern: Expr2627
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| Expr2627 | Expression_of | Gene | WBGene00006917 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006917 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000018 | ||||
| WBls:0000019 | ||||
| WBls:0000020 | ||||
| WBls:0000017 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0005733 | Certain | ||
| WBbt:0005812 | Certain | |||
| GO_term | GO:0005886 | |||
| GO:0005737 | ||||
| Subcellular_localization | Subcellular localization of vha-8 by immuno-gold electron microscopy using anti-VHA-8 antibodies detected signals in the cytoplasm of the excretory canals and stacked sheets of the plasma membranes of the syncytial hypodermal cells. | |||
| Type | Antibody | Rabbit polyclonal antibody against recombinant protein. The full-length vha-8 cDNA subcloned in pGEX-4T1 was over-expressed as glutathione S-transferase (GST) fusion protein in BL21 (DE3) E. coli strain, and the fusion protein was used to raise polyclonal antibodies in rabbit. | ||
| Pattern | Antibody staining patterns were consistent with the vha-8::GFP reporter gene expression pattern through all developmental stages, beginning at comma stage embryo to adult. Furthermore, vha-8 expression in canals of the H-shaped excretory cell, was prominently observed in a magnified image. Presumably, VHA-8 is expressed in syncytial hypodermal cells as well as in the excretory cell. | |||
| Picture | WBPicture0000012288 | |||
| Reference | WBPaper00006007 | |||
| Antibody_info | WBAntibody00000656 |
