WormBase Tree Display for Expr_pattern: Expr2580
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| Expr2580 | Expression_of | Gene | WBGene00000591 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00000591 | |||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000015 | ||||
| WBls:0000021 | ||||
| WBls:0000010 | ||||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0004017 | Certain | ||
| GO_term | GO:0005694 | |||
| Subcellular_localization | COH-1 was found in association with chromosomes regardless of the stage of the cell cycle in all the cells expressing it. | |||
| Type | Antibody | Affinity purified rabbit and rat polyclonal antibodies against recombinant COH-1. The relatively unique region in the open reading frame (amino acids 173559 for COH-1) was polymerase chain reaction amplified from a cDNA clone and cloned into pGEX-KG vector. Glutathione S-transferase (GST) fusion protein was expressed from the resulting plasmid in Escherichia coli and used as an antigen after purification. COH-1specific antibodies raised in rabbit and rat were affinity purified using histidine (His)-tagged COH-1. Both rabbit and rat antibodies were used in immunofluorescence analysis, and they gave the same staining patterns. | ||
| Pattern | COH-1 was not detected in early embryos. The COH-1 protein emerged in virtually all cells in embryos after the 100-cell stage and seemed to be expressed most strongly in embryos undergoing morphogenesis. During larval development, COH-1 could be seen apparently in all somatic cells, although the signal in the intestinal cells was somewhat weaker compared with other cells. Interestingly, however, COH-1 was not detected in germ cells throughout the larval development. | |||
| Picture | WBPicture0000008735 | |||
| Reference | WBPaper00005937 | |||
| Antibody_info | WBAntibody00000643 | |||
| WBAntibody00000644 |
